N. benthamiana seeds are planted in hydroponic media and transferred to trays for gravity fed loading into LED lit vertical farming racks. Our plants are grown indoors under tightly controlled conditions for consistency & quality. Seeding & Growth Seedlings are transplanted from germination trays to larger grow trays. 1 Vector Gene ofInterest Gene Cloning We synthesize the gene, encoding the desired protein and clone it into our high-productivity FastPharming Vectors. Mobilization The gene vectors are then loaded into agrobacteria, which are ideally suited to infect the N. benthamiana plants.
2 PlantInfiltration We then submerge the plants under a vacuum in a solution containing the agrobacteria to infiltrate the microbes into the leaves, where they then deliver the gene vectors into the plant cells. plant cells
The vectors instruct the plant cells to start producing an abundance of the desired protein, converting each plant into a small factory! ProteinProduction 3
Harvesting After about a week of growth, we harvest the plant leaves and their cargo of recombinant protein. 4
We process the leaves to extract the "green juice," that then undergoes industry standard protein purification before finishing the biologic in its final form. Purification & Vialing 5