Peptide-MHC tetramer technology description BIOTIN MHC PEPTIDE TAG STREPTAVIDIN Streptavidin molecules are labeled with a metal or fluorochrome tag and added to a solution of biotinylated peptide MHC monomers. Four peptide-MHC monomers bind to one tagged streptavidin molecule resulting in a peptide-MHC tetramer complex. BIOTIN MHC PEPTIDE STREPTAVIDIN TAG Unique Metal Barcoding Technology By combining three different labeled streptavidin molecules a unique triple barcode is generated... the peptide-MHC tetramers result in a unique triple metal code per peptide for detection by mass cytometry Choosing 3 metals out of 10 metals provides up to 120 unique triplet combinations METAL 1 METAL 2 METAL 3 METAL 4 METAL 5 METAL 6 METAL 7 METAL 8 METAL 9 METAL 10 Metal barcode #11 Metal barcode #10 Metal barcode #9 Metal barcode #8 Metal barcode #7 Metal barcode #6 Metal barcode #5 Metal barcode #4 Metal barcode #3 Metal barcode #2 Metal barcode #1